Effectiveness Test of Okra Fruit (Abelmoschus esculentus) Extract on The Growth of Trichophyton rubrum
Uji Efektivitas Ekstrak Buah Okra (Abelmoschus esculentus) Terhadap Pertumbuhan Jamur Trichophyton rubrum
-
(1) Bella Tasya Kimberly Prodi Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Sidoarjo
Indonesia
(2) * Chylen Setiyo Rini
Prodi Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Sidoarjo
Indonesia
(*) Corresponding Author
Abstract
Dermatophytosis is an infectious disease of the skin caused by one of the pathogenic agents of the fungus Trichophyton. Trichophyton rubrum is a dermatophyte fungus that is included in the anthropophilic classification, where transmission occurs through direct human-to-human contact. This experiment was conducted to determine the effectiveness of giving okra fruit extract (Abelmoschus esculentus) to the growth of Trichophyton rubrum using well method, media that is used to do that is SDA (Sabouraud Dextrose Agar) with various concentrations of 25 %, 50%, 75%, 100%. Based on the results of phytochemical tests, the active compounds contained in okra fruits extract are alkaloids, flavonoids, saponins, steroid, triterpenoids, phenolics and tannins. Variations in concentration of okra fruit extract and negative control tested against Trichophyton rubrum did not show the formation of inhibition zones in the media. Meanwhile for positive control using 2% ketoconazole, an inhibition zone 35 mm was formed. This research showed that Trichophyton rubrum was resistant to okra fruit extract.
References
Abdurrahman, A. F., Puspitaningrum, I., & Sari, W. K. (2021). Uji Aktivitas Gel Ekstrak Etanol Buah Okra (Abelmoschus esculentus) Terhadap Penyembuhan Luka Bakar Derajat II Kulit Punggung Kelinci Jantan Galur New Zealand. Cendekia Eksakta, 6(1), 29–35. doi: doihttps://doi.org/ 10.31942/ce.v6i1.4407
Andriani, C.R., Oesman, F., & Nursanty, R. (2016). Uji Zona Hambat Ekstrak
Etil Asetat Daun Alpukat (Persea americana Mill.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus. Jurnal Kedokteran Syiah Kuala, 16(1), 1-5. Diakses dari http://www.jurnal.unsyiah.ac.id/JKS/article/view/3936
CLSI. (2018). Method for Antifungal Disk Diffusion Susceptibility Testing of
Yeast 3rd ed. Wayne, PA: Clinical and Laboratory Standards Institute.
Dewi, S., Asseggaf, S. N., & Natalia, D. (2019). Artikel Penelitian Efek
Ekstrak Etanol Daun Kesum (Polygonum minus Huds.) sebagai Antifungi Terhadap Trichophyton rubrum. Jurnal Kesehatan Andalas, 8(2), 198–203. doi: https://doi.org/10.25077/jka.v8i2.992
Farihatun, A., Nurmalasari, A., Hayati, E., Sumirah, M., Setiawan, D., & Wahlanto, P. (2018). Identifikasi Jamur Penyebab Tinea Pedis Pada Kaki Penyadap Karet Di PTPN VIII Cikupa Desa Cikupa Kecamatan Banjar Sari Kabupaten Ciamis Tahun 2017. The Journal Of Medical laboratory, 6(7), 56–60. doi: https://doi.org/10.33992/m.v6i1.236
Gomez, B.L., & Nosanchuk J. D. (2003). Melanin and Fungi. Current Opinion in Infectious Diseases, 16(2), 91-96. doi: https://doi.org/10.1097/00001432-200304000-00005
Handayani, H., Sriherfyna, F. H., & Yunianta. (2016). Ekstraksi Antioksidan Daun Sirsak Metode Ultrasonic Bath (Kajian Rasio Bahan : Pelarut Dan Lama Ekstraksi). Jurnal Pangan dan Agroindustri, 4(1), 262-272. Diakses dari https://jpa.ub.ac.id/index.php/jpa/article/view/327/338
Hidayat, R. (2018). Hubungan Kebersihan Diri (Personal Hygiene) Dengan Kejadian Penyakit Dermatofitosis Di Desa Lereng Wilayah Kerja Puskesmas Kuok. Jurnal Ners, 2(1), 86-94. doi: https://doi.org/10.31004/jn.v2i1.713
Kar, B., Patel, P., & Free, S. J. (2019). Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins. PloS ONE, 14(4), 1-19. doi: https://doi.org/10.1371/journal.pone.0215034
Kementerian Kesehatan Republik Indonesia. (2011). Profil Kesehatan Indonesia 2010. Jakarta : Kementerian Kesehatan Republik Indonesia.
Pradita, R. A. & Wardani, A.N. (2019). Crash Course Dermatologi Dan Venereologi. Jakarta : Elsevier.
Rumampuk, Y. B. J., Wowor, P. M., & Mambo, C. D. (2017). Uji Daya Hambat Ekstrak Spons Laut (Callyspongia aerizusa) terhadap Pertumbuhan Bakteri Salmonella thypi dan Streptococcus pyogenes. Jurnal e-Biomedik. 5(2). doi: https:/doi.org/10.35790/ebm.v5i2.18480
Sinawe, H., & Casadeus, D. (2022). Ketoconazole. Treasure Island: StatPearls Publishing.
Solomon, S., Muruganantham, N., & Senthamilselvi, M. M. (2016). Antimicrobial activity of Abelmoschus esculentus (flowers). International Journal of Herbal Medicine, 4(6), 46–49. Retrieved from https://www.florajournal.com/archives/2016/vol4issue6/PartA/5-1-20-776.pdf
Surekha, A., Kumar, Gr., Sridevi, K., Murty, D., Usha, G., & Bharathi, G. (2015). Superficial dermatomycoses: A prospective clinico-mycological study. Journal of Clinical and Scientific Research, 4(1), 7-15. doi: https://doi.org/10.15380/2277-5706.jcsr.14.051
Tedjo, M. H. (2015). Uji Aktivitas Antijamur Infusa Daun Mangga Bacang (Mangifera foetida L.) Terhadap Pertumbuhan Jamur Trichophyton Rubrum. Skripsi. Universitas Tanjungpura. Pontianak.
Youngchim, S., Pornsuwan, S., Nosanchuk J. D., Dankai, W., & Vanittanakom, N. (2011). Melanogenesis in dermatophyte species in vitro and during infection. Microbiology, 157(Pt 8), 2348-2356. doi: https://doi.org/10.1099%2Fmic.0.047928-0
