Effectiveness of The Antibacterial Activity on Orthosiphon aristatus Leaves Extract Against Proteus mirabilis and Staphylococcus saprophyticus Uji Efektivitas Antibakteri Ekstrak Daun Kumis Kucing (Orthosiphon aristatus) Terhadap Bakteri Proteus mirabilis dan Staphylococcus saprophyticus
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Abstract
Orthosiphon aristatus is traditional medicinal plants. Orthosiphon aristatus leaves contain alkaloid, saponin, steroid, triterpenoid, and phenolic which are antibacterial compounds. Proteus mirabilis and Staphylococcus saprophyticus can cause urinary tract infections. This study aims to determine the optimum concentration of Orthosiphon aristatus leaves extract against Proteus mirabilis and Staphylococcus saprophyticus. This research is an experimental study. This research was carried out in the Bacteriology laboratory of the Muhammadiyah University of Sidoarjo. Antibacterial activity test of Orthosiphon aristatus leaves extract used the disc diffusion method at a concentration of 25%, 50%, 75%, 100%. This test is performed 3 times. Measurement of the diameter of the zone of inhibition is measured using a caliper. The data obtained were tested statistically with Anova with a significant level of 0.05 (5%). The results showed that the Orthosiphon aristatus leaves at a concentration of 25%, 50%, 75%, 100% had a significant effect on Proteus mirabilis with an average of 6.22 mm, 9.36 mm, and 15.55 mm, 21.22 mm and Staphylococcus saprophyticus with a mean of 5.4 mm, 8.8 mm, 14.53 mm, and 20.71 mm.
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Fadillah, Fairuz. M. J., Suharni, M., dan Wan, N. S. S. (2014) Antibacterial effect of banana pulp extracts based on different extraction methods against selected microorganisms. Asian Journal of Biomedical and Pharmaceutical Sciences. 04(36): 14-19. doi : 10.15272/AJBPS.V4I36.553
Ho, C. H., Noryati, I., Sulaiman, S. F., dan Rosma, A. (2010). In vitro antibacterial and antioxidant activities of Orthosiphon stamineus Benth. Methanol extracts against food-bornebacteria. Food Chemistry 122 :1168-1172
Morales, G., Sierra, P., Mancilla, A., Paredes, A., Loyola, L. A., Gallardo, O., dan Borquez, J. (2003). Secondary metabolites from four medicinal plants from northen chile : Antimicrobial Activity and Biotoxicity Against Artemia salina. Journal of The Chilean Chemical Society. 48(2):13-18. doi: 10.4067/S0717-97072003000200002
Ningsih, N. F., Ratnasari, E., dan Faizah, U. (2016). Pengaruh Ekstrak Daun Kumis Kucing (Orthisiphon aristatus) terhadap Mortalitas Hama Wereng Coklat (Nilaparvata lugens). LenteraBio Vol. 5 No. 1, 14-19. Retrieved from: https://jurnalmahasiswa.unesa.ac.id/index.php/lenterabio/article/view/14554
Suta, A. M. (2014). Aktivitas antibakteri ekstrak etanol daun Kumis Kucing (Orthosiphon Stamineus Benth)) terhadap Klebsiella pneumonia. ETD Universitas Syiah Kuala. Retrieved from: https://etd.unsyiah.ac.id/index.php?p=show_detail&id=4670
Syarifah, R., Fakhrurrazi, Harris, A., Sutriana, A., Erina, dan Winaruddin. (2018). Uji daya hambat ekstrak biji buah pala (Myristica fragrans Houtt) terhadap pertumbuhan bakteri Escherichia coli. Jurnal Mahasiswa Veteriner, 2(3). doi: 10.21157/jim%20vet..v2i3.8203
Toy, T. S. S., Benedictus, S., Lampus., Bernat, S.P., dan Hutagalung. (2015). Uji daya hambat ekstrak rumput laut Gracilaria sp tehadapat pertumbuhan bakteri Staphylococcus aureus. Jurnal e-GiGi(eG). 3 (1): 153-9 doi: doi: 10.35790/eg.3.1.2015.6600
Widiyastuti, Y. (2015). Pedoman budidaya panen dan pascapanen tanaman obat. Jakarta: Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan.
World Health Organization. (WHO). (2013). Kesehatan reproduksi wanita infeksi saluran kemih (ISK). Jakarta: Salemba Medika.