Kinerja Fungsi Ginjal pada Kelinci (Lepus nigricollins) Diabetes yang diberi Ekstrak Bonggol Buah Nanas (Ananas comusus L.)


Performance of Kidney Function in Rabbits (Lepus nigricollins) Diabetic given Pineapple (Ananas comusus L.) Weevil Extract


  • (1) * Very Rahmayanti            Universitas Muhammadiyah Sidoarjo  
            Indonesia

  • (2)  Syahrul Ardiansyah            Universitas Muhammadiyah Sidoarjo  
            Indonesia

    (*) Corresponding Author

Abstract

Bromelain enzyme contained in pineapple (Ananas comosus L.) are enzyme that have a function as anti-inflamatory in diabetic treatment periodically, will be giving impact to kidney function performance. This study to determine the effect of pineapple hump extract (Ananas comosus L.) on creatinine level in diabetic rabbits.  Rabbits were acclimated for 3 weeks than measured the creatinine level to know the first level. So, it induced by alloxan as much as 195 mg/kg during ±2 weeks for conditioning under hyperglicemia.  Then measured the creatinine level from 6 groups, it given pineapple extract with different concentration, those are positive control (+), negative control (-), extract 25%, 50%, 75% and 100%. Then measured of creatinine level did in enzymatic photometric method. In group of concentration 25% the creatinine level from 0,58 mg/dl become 1,07 mg/dl, in concentration 50% from 1,55 mg/dl become 1,25 mg/dl, in concentration 75% from 1,63 mg/dl become 1,19 mg/dl and in concentration 100% from 1,58 mg/dl become 1,42 mg/dl. The result of the study were tested statiscally with one way Anova test, it showed p> 0,05. It show Pineapple hump extract has no effect on the role of diabetic rabbits kidney.

References

Bala, M., Ismail, N. A., Mel, M., Jami, M. S., Saleh, H. M., & Amid, A. (2012). Bromelain Production: Current Trends And Perspective. Archives Des Sciences, (65)11, 369-399. Retrieved from http://irep.iium.edu.my/28364/1/Bromelain_review.pdf

Borrelli, F., Capasso, R., Severini, B., Fiorino, F., Aviello, G., ... Izzo, A. A. (2011). Inibitory Effect of Bromelin, a Cysteine Protease Derived from Pineapple Stem (Ananas comusus), on Intestinal Motility in Mice. Neurogastroenterol Motil, 23(8), 745-e331. doi: 10.1111/j.1365-2982.2011.01735.x

Fitzhugh, D. J., Shan, S., Dewhirst, M. W., & Hale, L. P. (2008). Bromelain treatment decreases neutrophil migration to sites of inflammation. Clinical Immunology, 128(1), 66-74. doi: 10.1016/j.clim.2008.02.015

Dyah, P. (2014). Diagnosis dan Klasifikasi Diabetes Melitus. Dalam: Buku Ajar Ilmu Penyakit Dalam. Jakarta: Pusat Penerbit FKUI.

Hendromartono. (2014). Nefropati Diabetik. Dalam: Buku Ajar Ilmu Penyakit Dalam. Jakarta: Pusat Penerbit FKUI.

Herdyastuti, N. (2006). Isolasi dan Karakterisasi Ekstrak Kasar Enzim Bromelin dari Batang Nanas (Ananas comusus L.merr). Berk. Penel. Hayati. Vol. 12, 75–77. Retrieved from http://berkalahayati.org/file/journals/1/article/487/submission/487-1606-1-sm.pdf

Ladhams, A., Scarnto, P., & Engwerda, C. (1999). Bromelain from Pineapple Stems Proteolytically Blocks Activation of Extracelluler Regulated Kinase-2 in T Cells. J Immunol. 163(5), 2568-2575. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/10452995.

Malole, M.M.B., Pramono. (1989). Penggunaan Hewan–Hewan Percobaan Laboratorium. Bogor: IPB. Ditjen Pendidikan Tinggi Pusat Antar Universitas Bioteknologi.

Naiola, E., N. Widhyastuti. (2007). Semi Purifikasi dan Karakterisasi Enzim Protease Bacillus sp. Berk Penelitian Hayati. 13: 51-56. Retrieved from http://berkalahayati.org/file/journals/1/article/452/submission/452-1463-1-sm.pdf

Perkumpulan Endokrinologi Indonesia. (2006). Diagnosis dan Konsensus Pengelolaan Diabetes Melitus di Indonesia. Jakarta: PB PERKENI.

Pavan, R., Jain, S., Shraddha., Ajay Kumar. 2012. Properties and Therapeutic Application of Bromelain: A Review. Hindawi Publishing Corporation Biotechnology Research International. doi: 10.1155/2012/976203.

Prince, A., & Wilson, L. (1995). Patofisiologi. Buku 2. Penerbit EGC. Jakarta.

Soegondo, S., Soewondo, P., Subekti, I., Oemardi, M., Semiardji, G., Soebardi, S. (2002). Petunjuk Praktis: Pengelolaan Diabetes Mellitus Tipe 2. Jakarta: PB Perkeni.

Suyono, S. (2007). Patofisiologi Diabetes Melitus. Dalam Penatalaksanan Diabetes Melitus Terpadu. Jakarta: Balai Penerbit FKUI.

Tochi, B. N., Zhang, W., Ying, X., & Wenbin, Z. (2008). Theraupetic Application of Pineapple Protease (Bromelain): A Review. Bromelain’s Pharmacology. Pakistan Journal of Nutrition, 7(4), 513-520. Retrieved from http://docsdrive.com/pdfs/ansinet/pjn/2008/513-520.pdf

Wien, M. W., & Sundari, D. (2010). Uji Toksisitas Sub Kronik Ekstrak Daun Kembang Sungsang (Gloriosa super L.) Terdapat Fungsi Ginjal Tikus Putih. Buletin Penelitian Kesehatan, 38(4), 186-191. Retrieved from http://media.neliti.com/pdfs/ansinet/pjn/2010/513-520.pdf

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Published
2019-12-31
 
How to Cite
Rahmayanti, V., & Ardiansyah, S. (2019). Kinerja Fungsi Ginjal pada Kelinci (Lepus nigricollins) Diabetes yang diberi Ekstrak Bonggol Buah Nanas (Ananas comusus L.). Medicra (Journal of Medical Laboratory Science/Technology), 2(2), 63-67. https://doi.org/10.21070/medicra.v2i2.1652
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Articles

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