The Effect Of Centrifugation Speed On The Results Of Urine Sediment Examination
Hubungan Pengaruh Kecepatan Sentrifugasi Terhadap Hasil Pemeriksaan Sedimen Urine
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(1) Muslimah Uswatun Hasanah Universitas Muhammadiyah Sidoarjo
Indonesia
(2) * Puspitasari Puspitasari
Prodi Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Sidoarjo
Indonesia
(*) Corresponding Author
Abstract
The process of centrifugation in urine examination is very important because it can be used in the manufacture of urine sediment. The purpose of this study was to determine the effect of centrifugation speed on the results of urine sediment examination. Centrifugation speeds used were 3000 rpm, 3500 rpm, 4000 rpm, and 4500 rpm using pathological urine samples. The samples used were 32. The research method used was an experimental laboratory at the Pusura Yos Sudarso clinical laboratory in Surabaya. The results of the study with a speed of 3000 rpm obtained an average of erythrocytes = 6.63/LPB, leukocytes = 13.63/LPB, and epithelial = 10.50/LPB. At a speed of 3500 rpm the average value of erythrocytes = 7.63/LPB, leukocytes = 15.13/LPB, and epithelium 11.63/PLB. At a speed of 4000 rpm the average value of erythrocytes = 8.63/LPB, leukocytes = 17.75/LPB and epithelial = 13.50/LPB. At a speed of 4500 rpm the average value of erythrocytes = 9.63/LPB, leukocytes = 20.50/LPB, and epithelial = 15.38/LPB. The results of the statistical test One Way Anova obtained p-value results of 0.001 < (0.05) in erythrocyte cells, p-value results for leukocytes 0.025 < (0.05), and p-value results for epithelial cells 0.002 < (0.05) means that there is an effect on the centrifugation speed on the results of urine sediment examination.
References
Ingrat, I. W. (2018). Pengaruh Kecepatan Pemusingan Terhadap Jumlah Leukosit Urin Metode Manual. Skripsi. Universitas Muhammadiyah Semarang. Semarang. Retrieved from http://repository.unimus.ac.id/3174/
Liseike, C. L., & Zeibig, E. A. (2018). Laboratorium Klinis. Jakarta: EGC.
Nugraha, C., Ardiansah., Ardiansah, H., & Aswad, H. (2019). Pengaruh Lama Sentrifugasi Sampel Urine Terhadap Hasil Pemeriksaan Sedimen Lekosit Urine Pada Penderita Infeksi Saluran Kemih (ISK) di Laboratorium D-III Analis Kesehatan Universitas Indonesia Timur Tahun 2019. Jurnal Media Laboran, 9(2), 6-12. doi: 10.29407/jbp.v6i2.14793.
Sabban, I. F., Wahyuni, I. N., Erawati E., Hermawan R. A., Nela, F. V., Kurniawan, A. E., & Anggraini, E. R. (2020). Pengaruh Kecepatan dan Waktu Sentrifugasi Terhadap Sedimentasi pada Pembuatan Sediaan dalam Pemeriksaan Mikroskopis Urin. Prosiding Seminar Hasil Penelitian 2020. IIKB W Press. Retrieved from https://prosidingonline.iik.ac.id/index.php/PSHP/article/view/152
Siti, S. C. (2020). Optimasi Sentrifugasi Terhadap Jumlah Sel Epitel Pada Pemeriksaan Sedimen Urine. Skripsi. Politeknik Kesehatan Kemenkes Bandung. Bandung. Retrieved from https://repo.poltekkesbandung.ac.id/1431/
Payadnya, I. P. A. A., & Jayantika, I. G. A. T. (2018). Panduan Penelitian Eksperimen Beserta Analisis Statistik dengan SPSS. Yogyakarta: CV Budi Utama.
Yanti, D. (2019). Identifikasi Jumlah Sedimen Urine Pada Penderita Infeksi Saluran Kemih Di Puskesmas Arosbaya. Karya Tulis Ilmiah. STIKes Ngudia Husada Madura. Bangkalan. Retrieved from http://repository.stikesnhm.ac.id/id/eprint/179/
