Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus
Efektivitas Ekstrak Etanol Daun Lidah Buaya (Aloe vera) terhadap Staphylococcus aureus
Abstract
Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations
References
Ajizah, A. (2004). Sensivitas Salmonella typium Terhadap Ekstrak Daun Pisidium guajava L. Bioscientiae, 1(1), 31-38. Retrieved from https://ppjp.ulm.ac.id/journals/index.php/bioscientiae/article/view/130
Brooks, G. F, Butel, J.S., Carrol, K. C., & Morse, S. A. (2007). Mikrobiologi Kedokteran. 23th Ed. Jakarta: EGC.
Chantin, A., & Suharto. (1994). Sterilisasi dan Desinfektan dalam Mikrobiologi Kedokteran, Edisi Revisi. Jakarta: Binarupa Aksara.
Furmawanthi, I. (2002). Khasiat & Manfaat Lidah Buaya Si Tanaman Ajaib. Jakarta: Argo Media Pustaka. Hal 1-21.
Rieuwpassa, I. E., Rahmat, & Karlina. (2011). Daya Hambat Ekstrak Aloe vera terhadap pertumbuhan Staphylococcus aureus. Dentofasial, 10(2), 65-70. Retrieved from https://jdmfs.org/index.php/jdmfs/article/viewFile/256/256
Natsir, N. A. (2013). Pengaruh Ekstrak Daun Lidah Buaya (aloe vera) sebagai penghambat pertumbuhan Bakteri Staphylococcus aureus. Prosiding FMIA Universitas Patimura. 110-112. Retrieved from https://ejournal.unpatti.ac.id/ppr_iteminfo_lnk.php?id=509
Pandey, R. & Avinash, M. (2010). Antibacterial Activites of Crude Extract of Aloe barbadonsis of Clinically Isolated Bacterial Phatogen. Appl Biochem Biotechnol, 160(5), 1356-1361. doi: 10.1007/s12010-009-8577-0 DOI: https://doi.org/10.1007/s12010-009-8577-0
Pratama, M. R. (2005). Pengaruh Ekstrak Serbuk Kayu Siwak (Saivadora persica) Terhadap Pertumbuhan Bakteri Streptococcus mutan dan Staphylococcus aureus Dengan Metode Difusi Agar. Skripsi. Institut Pertanian Bogor. Bogor.
Schelegel, H. G. (1994). Mikrobiologi Umum. Edisi keenam. Yogyakarta: Gajah Mada University Press.
Tammi, A., Apriliana, E., Sholeha, T. U., & Ramadhian, M. R. (2018). Inhibition Potential of Bay Leaf Extract (Syzygium polyanthum [Wight.] Walp.) as Antibacterial to Staphylococcus aureus In Vitro. J Agromedicine Unila, 5(2), 562-566. Retrieved from http://repository.lppm.unila.ac.id/12695/1/2113-2832-1-PB.pdf
Yusitta, Y. (2018). Efektivitas Ekstrak Daun Lidah Buaya terhadap Pertumbuhan Staphylococcus aureus dengan metode Difusi. Karya Tulis Ilmiah. STIKES ICME Jombang.