The Potential of Mango (Mangifera infica L.) Peel of Apple Varieties By Infusion And Maceration In Inhibiting Pseudomonas aeruginosa And Propionibacterium acnes
Potensi Kulit Mangga (Mangifera infica L.) Varietas Apel Secara Infusa Dan Maserasi Dalam Menghambat Bakteri Pseudomonas aeruginosa dan Propionibacterium acnes
Abstract
Plants have many chemical components. The use of natural ingredients as an alternative treatment in dealing with diseases, especially acne. One of them is mango (Mangifera indica L.) varieties of apples obtained at the Larangan Main Market in Sidoarjo. This study aims to determine the potential of infusion and maceration of mango skin varieties in inhibiting Pseudomonas aeruginosa and Propionibactrium acne at various concentrations. This antibacterial potential test was carried out using the diffusion method of the wells. The antibacterial potential is characterized by the formation of a clear zone around the well called the inhibition zone. This study uses 10 concentrations namely 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% and Clindamycin as positive control and aquades as negative control. Based on the results of the Two Way ANOVA test data obtained were not normally distributed, therefore a comparison test was performed using the Kruskal-Wallis test with a sign value (α <0.05). This showed that there were significant differences in the use of various concentrations. The maceration extract concentration of 100% is the best concentration to form a zone of inhibition against P. aeruginosa of 17.9 mm and P. acne bacteria of 13.2 mm. The results of the infusion extract concentration did not form inhibitory zones in both of P. aeruginosa and P. acnes.
References
Choffnes, E. R. (2010). Antibiotic Resistance. The National Academic Press.
Clinical and Laboratory Standart Institute (CLSI). (2013). Performance Standart for Antimicrobial Disk Suspecbility Test: Approved Standart Eleventh Edition.Wayne.
Fissy, A.S.O.N., Sari, R., & Pratiwi, L. (2014). Uji efektifitas sediaan gel anti jerawat ekstrak etanol rimpang jahe merah (Zingiber officinale Rosc. var. Rubrum) terhadap Propioni bacterium acnes dan Staphylococcus epidermidis. Jurnal Ilmu Farmasi Indonesia, 12(2), 193-201. Retrieved from https://garuda.ristekbrin.go.id/documents/detail/955576
Handa, S. (2008). Extraction tecnologies for medical and aromaticplants. Trieste: International Centre For Science and High Tecnology.
Hussain, H. T. (2018). Estimation of Antibacterial Activity of Green Mango (Mangifera indica L) Extract on the Growth of Bacteria. AlMustansiriyah Journal of Science, 29(1), 75-78. doi: 10.23851/mjs.v29i1.113. DOI: https://doi.org/10.23851/mjs.v29i1.113
Ismarani, D., Pratiwi, L., & Kusharyanti, I. (2014). Formulasi gel pacar air (Impatiens balsamina Linn.) terhadap Propionibacterium acnes dan Staphylococcus epidermidis. Pharm Sci Res, 1(1), 30-45. doi: 10.7454/psr.v1i1.3504 DOI: https://doi.org/10.7454/psr.v1i1.3504
Jeong, S.M., Kim, S.Y., Kim D.R., Jo, S.C., Nam, K.C., Ahn, D.U., & Lee, S. (2004). Effect of heat treatment on the antioxidant activity of extracts from Citrus peels. Jurnal Agric. Food Chem, 52(11), 3389-3393. doi:10.1021/jf049899k. DOI: https://doi.org/10.1021/jf049899k
Munawaroh, Z. F., Aufia, W., & Masitha, N. (2017). Uji Aktivitas Antibakteri Ekstrak Ethanol Biji Mangga (Mangifera indica L.) Terhadap Propionibacterium acnes. Pharmasipha, 1(1), 31-35. doi: 10.21111/pharmasipha.v1i1.1122 DOI: https://doi.org/10.21111/pharmasipha.v1i1.1122
Sandhu, K. S., &. Lim, S. T. (2008). Structural characteristics and in vitrodigestibility of mango kernel starches (Mangifera indica L). J. Food Chem, 107(1), 92-97. Retrieved from https://koreauniv.pure.elsevier.com/en/publications/structural-characteristics-and-in-vitro-digestibility-of-mango-ke DOI: https://doi.org/10.1016/j.foodchem.2007.07.046
Waluyo, L. (2007). Mikrobiologi Umum. Malang: UMM Press.
Wasitaatmadja, S.M. (2011). Dermatologi kosmetik penuntun ilmu kosmetik Medik. Edisi kedua. Jakarta: Fakultas Kedokteran Universitas Indonesia.
Wyatt, H., Patel., G.K., Kubiak, E.M., Clark S.M., & Mills, C.M. (2001). Staphylococcus aureus colonization of children with atopic eczema and their parents. Acta Derm Venereol, 81(5):366-7. doi: 10.1080/000155501317140124 DOI: https://doi.org/10.1080/000155501317140124