The Effect of Delayed Examination and Storage Conditions on Plasma Glucose Levels
Pengaruh Variasi Waktu Penundaan Pemeriksaan dan Kondisi Penyimpanan terhadap Kadar Glukosa Plasma
DOI:
https://doi.org/10.21070/medicra.v9i1.1831Keywords:
Delay Examination, EDTA Plasma, Glucose, NaF Plasma, Storage ConditionsAbstract
Blood glucose examination is an important parameter for screening and monitoring diabetes mellitus. The accuracy of glucose testing is influenced by pre-analytical factors, particularly delayed examination and specimen storage conditions. Delayed examination may cause a decrease in glucose levels due to ongoing glycolysis after blood collection. This study aimed to determine the effect of examination delay time and storage conditions on plasma glucose levels. This laboratory experimental study used a factorial design with repeated measures. The delay times consisted of 0, 2, and 4 hours, while the storage conditions included EDTA plasma stored at room temperature, EDTA plasma stored at 4ºC, and NaF plasma. Plasma glucose levels were measured using the GOD-PAP method. Data were analyzed using the Shapiro-Wilk test, Levene’s test, Two-Way ANOVA, and Tukey Post Hoc test. The results showed that plasma glucose levels decreased as the examination delay time increased. After 4 hours of delay inEDTA plasma at room temperature, glucose levels were reduced by 55.8%, EDTA plasma stored at 4ºC by 52.3%, and NaF plasma by 30.7%. There was a significant effect of variations in examination delay time and storage conditions on plasma glucose levels (p<0.005). It can be concluded that NaF plasma was more effective in maintaining glucose stability compared with EDTA plasma stored at room temperature or under refrigeration conditions.
References
Agarwal, A., Manjrekar, P. A., Ahmad, A., & Yadav, C. (2020). Effect of Time Interval between Collection , Plasma Separation and Analysis and the Effect of Temperature on the Laboratory Results of Plasma Glucose Estimation. International Journal of Biochemistry Research & Review, 29(2), 48–54. Doi: 10.9734/IJBCRR/2020/v29i230172
Fatmariza, A. R., Kurniawan, A. E., & Sari, N. (2024). Stabilitas Pooled Sera Etilen Glikol dan Pooled Plasma NaF Sebagai Bahan Kontrol Pemeriksaan Glukosa. Jurnal Riset Pengembangan Dan Pelayanan Kesehatan, 3(2), 49–54. Doi: 10.56399/jgl.v3i2.218
Grzych, G., Defauwes, I., Tullio, P. De, Pekar, J. D., Lippi, G., Cavalier, E., Grzych, G., Defauwes, I., Tullio, P. De, & Pekar, J. D. (2025). Blood Glucose Measurement Inside and Outside the Laboratory: Both Preanalytical and Analytical Challenges. Critical Reviews in Clinical Laboratory Sciences, 62(8), 613–630. Doi: 10408363.2025.2533855
Gunawan, H. A., & Safari, W. F. (2024). Perbandingan Kadar Glukosa Darah Mengguankan Sampel Serum, Plasma Heparin dan Plasma NAF. Jurnal Wiyata, 11(01), 36–42. Doi: 10.56710/wiyata.v11i1.785
Gurung P, Zubair M, Jialal I. Plasma Glucose. [Updated 2024 Feb 27]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2026 Jan-. Available from: https://www.ncbi.nlm.nih.gov/sites/books/NBK541081/?utm_sou rce=chatgpt.com
Ibanga, I. E., Nwapi, Lucy Larrycarr Ekpo, Matina Imaobong Akpan, Hilary Andrew Abara, U. S. O. N., John, M., Joseph, Nduese Innocent Konglam, Nanle Ibrahim Dalen, M. B., & Nkwocha, B. C. (2023). The Impact of Delayed Analysis on Glucose Levels of. International Journal of Innovative Science and Research Technology, 8(2), 2–5. Doi: 10.5281/zenodo.7807393
Mulyani, E., Pangesti, I., & Farabi, M. F. (2023). Perbedaan Kadar Glukosa Darah Sewaktu menggunakan Antikoagulan Ethylene Diamine Tetra Acetic Acid (EDTA) dan Natrium Florida (NaF) dengan Variasi Penundaan Waktu Pemeriksaan pada Siswa SMK Semesta Bumiayu. Jurnal Ilmiah Kefarmasian, 5(2), 44–49. Doi: 10.36760/jp.v6i2.607
Nabilah, S., Khotimah, E., & Pramitaningrum, I. K. (2023). Pemantapan Mutu Internal Pra-Analitik pada Pemeriksaan Glukosa Darah Puasa di Laboratroium Duren Sawit. Binawan Student Journal (BSJ), 5(2), 58–64. Doi: 10.54771/bsj.v5i2.1127
Nurjanah, F., Nurhayati, D., Kurnaeni, N., & Riyani, A. (2023). Perbedaan Kadar Glukosa Darah Metode GOD-PAP pada Plasma Natrium Flourida (NaF) dengan Penundaan Waktu Pemeriksaan pada Suhu Ruang. Jurnal Kesehatan Poltekkes Palembang, 18(2), 187–193. Doi: 10.36086/jpp.v18i2.1892
Prasetyo, R., Wijayati, W., & Ayuningtyas, D. (2021). Identifikasi Waste Tahap Pra Analitik dengan Pendekatan Lean Hospital di Laboratorium Patologi Klinik RS XYZ Depok Jawa Barat Tahun 2021. Jurnal Manajemen Kesehatan Indonesia, 9(2), 102–112. Doi: 10.14710/jmki.9.2.2021.101-112
Rahmatunisa, A. N., Ali, Y., & Ms, E. M. (2021). Perbandingan Hasil Pemeriksaan Glukosa Darah pada Serum Segera dan Ditunda Selama 24 Jam. Prepotif Jurnal Keseahatan Masyarakat, 5(2), 1180–1185. Doi: 10.31004/prepotif.v5i2.2112
Sacks, D. B., Arnold, M., Bakris, G. L., Bruns, D. E., Horvath, A. R., & Lernmark, Å. (2023). Guidelines and Recommendations for Laboratory Analysis in the Diagnosis and Management of Diabetes Mellitus BACKGROUND : SUMMARY : Clinical Chemistry, 69(8), 808–868. Doi: 10.1093/clinchem/hvad080
Sinaga, H., & Irianti, C. (2020). Perbandingan Hasil Pemeriksaan Glukosa Darah Dengan Menggunakan Serum Dan Plasma Natrium Fluorida ( NaF ) Di Laboratorium Klinik Medika Jayapura. The Journal of Muhammadiyah Medical Laboratory Technologist, 3(1), 69–76. Doi: 10.30651/jmlt.v3i1.1999
Susiwati. (2018). Perbedaan Kadar Glukosa Darah Puasa Pasien Diabetes Melitus Tipe 2 pada Plasma NaF Berdasarkan Waktu Pemeriksaan di RSUD dr. M. Yunus Provinsi Bengkulu Tahun 2017. Journal of Nursing and Public Health, 6(1), 82–87. Retrieved from: View of Perbedaan Kadar Glukosa Darah Puasa Pasien Diabetes Melitus Tipe 2 Pada Plasma NaF Berdasarkan Waktu Pemeriksaan Di Rsud Dr. M.Yunus Provinsi Bengkulu Tahun 2017
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Yuliandi, N. E., Apriani, & Yaufita, L. (2022). Perbedaan Kadar GLukosa Darah dalam Plasma Natrium Flourida (NaF) dengan Penundaan Waktu Pemeriksaan. Klinikal Sains, 10(1), 10–16.Doi: 10.36341/klinikal_sains.v10i1.2165
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