Description of Wistar Rat (Rattus norvegicus) Skin Mast Cells with Hematoxylin Eosin and Alcian Blue Safranin Staining
Gambaran Sel Mast Kulit Tikus Wistar (Rattus norvegicus) dengan Pewarnaan Hematoxylin Eosin dan Alcian Blue Safranin
DOI:
https://doi.org/10.21070/medicra.v9i1.1812Keywords:
Alcian Blue Safranin, Hematoxylin Eosin, Mast Cells, Mouse Skin, StainingAbstract
One of the steps in making histological preparations is staining. Staining is a stage that aims to simplify the process of observing tissue structure and also makes it easier to distinguish the parts of the tissue that will be observed. Before staining, mast cells are difficult to identify visually under a microscope. Hematoxylin eosin is a routine stain in histotechnics and alcian blue safranin is a special dye that can stain mast cells. Mast cells can be found at the boundaries of body tissue and the external environment, one of which is the skin. Mast cells found in the skin are located in the dermis of the skin. The aim of this study was to determine the histology of skin connective tissue mast cells in Wistar rat (Rattus norvegicus) preparations stained with hematoxylin eosin and Alcian blue safranin. This research uses a experimental laboratory research type with a qualitative descriptive research design. The results obtained from observations of the histology of mast cells in the connective tissue of the skin of Wistar rats (Rattus norvegicus) with hematoxylin eosin staining from 16 preparations were found to be 62.5% good and 37.5% unfavorable. Staining with alcian blue safranin of 16 preparations was found to be 81.25% good and 18.75% unfavorable. The research results from preparations stained with alcian blue safranin showed better results compared to preparations stained with hematoxylin eosin.
References
Ariyadi, T., & Suryono, H. (2017). Kualitas sediaan jaringan kulit metode microwave dan conventional histoprocessing pewarnaan hematoxylin eosin. JLabMed, 1(1), 7–11. Retrieved from Kemendikdasmen https://share.google/DvdpcujkJHvqtXvTb
Elen, M. R. (2019). Gambaran Kualitas Mikroskopis Sediaan Hepar Mencit (Mus musculus ) dengan Pemotongan Ketebalan 2µm , 5µm dan 8µm . Jurnal Laboratorium Medis, 04(02), 71–78. Retrieved from Sumber: Poltekkes Kemenkes Semarang https://share.google/uvQf3HPnQa89vHm1c
Ellyawati, E. (2018). Penentuan Waktu Yang Tepat Pada Proses Staining Dalam Pembuatan Preparat Histologis Hati. Jurnal TEMAPELA, 1(1), 28–30. Retrieved from https://share.google/yaeIUrUktgFRkg72w
Ibrahim, M. N., Widjajanto, E., & Rosita, R. (2008). Distribution of Mast Cell in the Rat Mesentery. Jurnal Kedokteran Brawijaya, 24(10), 1–4. Retrieved from Jurnal Kedokteran Brawijaya https://share.google/TuLhdwieC5cyupGUD
Jusuf, A. (2009). Histoteknik Dasar. Jakarta: Universitas Indonesia, 1–33.
Kalangi, S. J. R. (2014). Histofisiologi Kulit. Jurnal Biomedik (Jbm), 5(3), 12–20. Doi: https://doi.org/10.35790/jbm.5.3.2013.4344
Nugroho, A. E., & Maeyama, K. (2011). Evaluasi Pewarnaan Alcian Blue Terhadap Sel Mast Jaringan Ikat dari Preparat Beku Jaringan Kulit Kaki Tikus. In Pharmacy, 8(2), 10–20. Retrieved from https://share.google/DBD8q8zzXh52BiQGS
Prasetiawan, E., Sabri, E., & Ilyas, S. (2013). Gambaran Histologis Hepar Mencit (Mus Musculus L.) Strain Ddw Setelah Pemberian Ekstrak N-Heksan Buah Andaliman (Zanthoxylum Acanthopodium Dc.) Selama Masa Pra Implantasi Dan Pasca Implantasi. Saintia Biologi, 1(1), 1–6. Retrieved from https://www.neliti.com/publications/221095/gambaran-histologis-hepar-mencit-mus-musculus-l-strain-ddw-setelah-pemberian-eks
Respati, L. A. (2020). Gambaran Fiksasi Organ Jaringan Pada Mamalia Menggunakan Aseton Dengan Variasi Waktu. 00(00). Retrieved from https://repository.poltekkes-smg.ac.id/index.php/index.php?p=show_detail&id=20893&keywords=
Safrida. (2012). Deteksi senyawa mukopolisarida dengan pewarnaan alcian blue pada ovarium dan uterus tikus putih rattus norvegicus. Jesbio, 1(1), 2302–1705. Retrieved from https://media.neliti.com/media/publications/76770-ID-deteksi-senyawa-mukopolisarida-dengan-pe.pdf
Sofyanita, E. N., & Annisa, A. S. (2023). Pengaruh Penggunaan Minyak Zaitun Dengan Pemanasan Sebagai Larutan Penjernih (Clearing) Terhadap Kualitas Sediaan Jaringan Hepar Mencit (Mus musculus). Jurnal Labora Medika, 7(1), 6-12. Retrieved from https://jurnal.unimus.ac.id/index.php/JLabMed/article/view/11795/pdf
Syafiq Naqsyabandi. (2022). Gambaran Variasi Waktu Pewarnaan Papanicolaou pada Preparat Sitologi Mukosa Mulut Perokok. Jurnal Medika Husada, 2(1), 19–24. Retrieved from Jurnal AAK Pekalongan https://share.google/SP5YOGcvpBu3h1tae
Wangko, S., & Karundeng, R. (2014). Komponen Sel Jaringan Ikat. Jurnal Biomedik (Jbm), 6(3), 1–7. doi: https://doi.org/10.35790/jbm.6.3.2014.6327
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