The Effectiveness Of a Combination Of Rosella (Hibiscus sabdariffa) and Red Ashoka (Ixora javanica) Extracts as an Eosin Substitute For Parasite Examination in Feces

Efektivitas Kombinasi Ekstrak Rosella (Hibiscus Sabdariffa) dan Asoka Merah (Ixora Javanica) Sebagai Pengganti Eosin Untuk Pemeriksaan Parasit pada Feses

Authors

  • Ahsanatul Munawwaroh Prodi D4 Analis Kesehatan, Fakultas Kesehatan, Universitas Nahdlatul Ulama Surabaya, Indonesia
  • Yauwan Tobing Lukiyono Prodi D4 Analis Kesehatan, Fakultas Kesehatan, Universitas Nahdlatul Ulama Surabaya, Indonesia
  • Irwan Sulisti Prodi Sarjana Terapan Sanitasi Lingkungan, Politeknik Kesehatan Surabaya, Indonesia
  • Agus Aan Adriansyah Prodi S1 Kesehatan Masyarakat, Fakultas Kesehatan, Universitas Nahdlatul Ulama Surabaya

DOI:

https://doi.org/10.21070/medicra.v8i2.1805

Keywords:

Eosin, Helminth Eggs, Red Ixora (Ixora javanica), Roselle (Hibiscus sabdariffa), Stool Examination

Abstract

Stool examination is an important diagnostic method for detecting intestinal helminths, particularly in tropical regions like Indonesia. Eosin 2% is commonly used to enhance microscopic visualization of helminth eggs but raises concerns regarding toxicity and environmental impact. This study aimed to evaluate the effectiveness of combining roselle (Hibiscus sabdariffa) and red ixora (Ixora javanica) flower extracts as a natural substitute for eosin in stool examination. This experimental research used a post-test-only control group design with four treatment groups: eosin (positive control), and three extract concentration combinations (60%:40%, 50%:50%, and 70%:30%). The results showed that all extract combinations produced comparable contrast and clarity to eosin in visualizing helminth eggs. The Kruskal–Wallis test indicated no significant difference between treatments (p>0.05). The combination of roselle and red ixora extract can therefore be considered a potential natural alternative to eosin for stool examination without reducing microscopic effectiveness.

References

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Published

2025-12-31

How to Cite

Munawwaroh, A., Lukiyono, Y. T., Sulisti, I., & Adriansyah, A. A. (2025). The Effectiveness Of a Combination Of Rosella (Hibiscus sabdariffa) and Red Ashoka (Ixora javanica) Extracts as an Eosin Substitute For Parasite Examination in Feces: Efektivitas Kombinasi Ekstrak Rosella (Hibiscus Sabdariffa) dan Asoka Merah (Ixora Javanica) Sebagai Pengganti Eosin Untuk Pemeriksaan Parasit pada Feses. Medicra (Journal of Medical Laboratory Science Technology), 8(2), 88–92. https://doi.org/10.21070/medicra.v8i2.1805

Issue

Vol. 8 No. 2 (2025): December

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Articles

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