Comparison of HCG Levels in Reagents Temperature 25°C and 8°C Using ELISA Method
Perbandingan Kadar HCG Pada Reagen Suhu 25°C dan 8°C Menggunakan Metode ELISA
-
(1) Prisca Audra Telleng Prodi D-IV Analis Kesehatan, Fakultas Ilmu Keperawan dan Kesehatan, Universitas Muhammadiyah Semarang
Indonesia
(2) * Budi Santosa
2Program Studi S2 Ilmu Laboratorium Klinis, Fakultas Ilmu Keperawatan dan Kesehatan, Universitas Muhammadiyah Semarang
Indonesia
(3) Aprilia Indra Kartika Prodi D-IV Analis Kesehatan, Fakultas Ilmu Keperawan dan Kesehatan, Universitas Muhammadiyah Semarang, Jawa Tengah, Indonesi
Indonesia
(*) Corresponding Author
Abstract
HCG hormone is a hormone produced by the placenta in early pregnancy with the aim of maintaining pregnancy. The Elisa method is a method for examining HCG levels which has the principle of specific antigen and antibody reactions. The work stage in the laboratory that has the biggest error is in the pre-analytic stage reaching 60%, one of which is the reagent temperature. The ELISA method recommends the reagent temperature to be stored at 2-8°C and when used at 25°C. Examination reagents Elisa method uses reagents that use enzymes in the examination. Enzymes are very sensitive to temperature. This study aims to determine the ratio of HCG levels at reagent temperatures of 25°C & 8° using the ELISA method. This type of research is analytic supported by experiments and literature studies. Serum samples of 6 pregnant women were taken from the Kedungmundu Public Health Center using a limited population count formula. The examination was carried out by comparing the reagents at a temperature of 25°C and 8°C using 6 serums of pregnant women and in duplicate. Data analysis only used primary data and statistical tests were carried out using normality, homogeneity and T-Test tests. The average result at a temperature of 25°C was 380.851 and the average temperature of 8°C was 321.99 and the T-test statistic test showed p value = 0.469 and 0.470. Based on the results of the study, it can be concluded that there is an insignificant difference in the concentration of HCG by comparing the reagents at 25°C and 8°C.
References
Aydin, S. (2015). A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides, Vol. 72, 4–15. doi: 10.1016/j.peptides.2015.04.012. DOI: https://doi.org/10.1016/j.peptides.2015.04.012
Betz, D., and Fane, K. (2022). Human Chorionic Gonadotropin. Treasure Island (FL): StatPearls Publishing. Retrieved from https://pubmed.ncbi.nlm.nih.gov/30422545/
Chemicon International, I. (2022). Elisa Reagents. Retrieved from http://www.elisa-antibody.com/ELISA-Introduction/elisa-reagents.html.
Dahlan, S. M. (2019). Statistik-Untuk-Kedokteran-Dan-Kesehatan-Msopiyudin-Dahlan_Compress.Pdf. Retrieved from https://doku.pub/download/statistik-untuk-kedokteran-dan-kesehatan-msopiyudin-dahlan-30j8pxk4p5lw.
Fahisyah, R. N., Naim, N. and Armah, Z. (2019). Pengaruh Variasi Lama Penyimpanan Reagen Enzim 1a Terhadap Hasil Pemeriksaan Ureum Darah Metode Berthelot. Jurnal Media Analis Kesehatan, 10(1), 21-27. doi: 10.32382/mak.v10i1.980. DOI: https://doi.org/10.32382/mak.v10i1.980
Flannery, J., Benzinger, M. J., Bird, J. P., Crowley, E. S., Goins, D., and Agin J. R. (2016). Validation of the Ridascreen ® Fast Milk Kit. Journal of AOAC international, 99(2), 495–503. doi: 10.570/jaoacint.15-0290. DOI: https://doi.org/10.5740/jaoacint.15-0290
Harti, A. S., Estuningsih., and Nurkusumawati, H. (2013). Pemeriksaan HCG (human chorionic gonadotropin) untuk deteksi kehamilan dini secara immunokromatografi. Jurnal Kesmadaska, 1(1), 1–4. Retrieved from https://jurnal.ukh.ac.id/index.php/JK/article/view/54/99
Jousselme, E., Jourdy, Y., Rugeri, L., Négrier, C., & Nougier, C. (2018). Comparison of an automated chemiluminescent assay to a manual ELISA assay for determination of von Willebrand Factor collagen binding activity on VWD plasma patients previously diagnosed through molecular analysis of VWF. Int J Lab Hematol, 40(1), 77-83. doi: 10.1111/ijlh.12743 DOI: https://doi.org/10.1111/ijlh.12743
Kustiningsih., Megawati, N., Kartiko, J. J., and Lutpiatina, L. (2017). Pengaruh Variasi Suhu Awal Reagen terhadap Kadar Glukosa Darah Metode Enzimatik. Medical Laboratory Technology Journal, 3(1), 103-107. doi: 10.31964/mltj.v3i1.147. DOI: https://doi.org/10.31964/mltj.v3i1.147
Madiana., and Rahayu, I. G. (2017). Pengangar Laboratorium Medis. Jakarta: Pusat Pendidikan Sumber Daya Manusia Kesehatan Badan Pengembangan dan Pemberdayaan Sumber Daya Manusia Kesehatan.
Marfia, N., Iqbal, M., and Juliatia, P. (2018). Identifikasi Hormon HCG pada Tes Kehamilan. Jurnal Ipb, 1(1), 35–55. Retrieved from https://www.academia.edu/37423528/IDENTIFIKASI_HORMON_HCG_PADA_TES_KEHAMILAN
Marliana, N., and Widhyasih, R. M. (2018). Bahan Ajar Teknologi Laboratorium Medik (TLM) Imunoserologi. Jakarta: Pusat Pendidikan Sumber Daya Manusia Kesehatan Badan Pengembangan dan Pemberdayaan Sumber Daya Manusia Kesehatan.
Nauly., Patricia, G., and Khairinisa, G. (2018). Panduan Analisis Laboratorium Imunoserologi untuk D3 Teknologi Laboratorium Medis. Cimahi: Stikes Jenderal Achmad Yani
Nurfia. (2016). Perbandingan Hasil Pemeriksaan Widal Menggunakan Reagen Yang Disimpan Di Suhu Ruangan Dengan Suhu Lemari Es Pada Penderita Suspeks Typoid Di RSUD Kota Kendari. Karya Tulis Ilmiah. Politeknik Kesehatan Kendari. Kendari.
Santosa, B. (2020). Teknik Elisa. Metode Elisa Untuk Pengukuran Protein Metallothionein Pada Daun Padi Ir Bagendit. Semarang: Unimus Press.
Shah, K., and Maghsoudlou, P. (2016). Enzyme-linked immunosorbent assay (ELISA): The basics. British Journal of Hospital Medicine, 77(7), C98–C101. doi: 10.12968/hmed.2016.77.7.C98. DOI: https://doi.org/10.12968/hmed.2016.77.7.C98
Siregar, M. T., Winke, S., Doni, S., & Anik, N. (2018). Bahan Ajar Teknologi Laboratorium Medik (TLM) Kendali Mutu. Jakarta: Pusat Pendidikan Sumber Daya Manusia Kesehatan Badan Pengembangan dan Pemberdayaan Sumber Daya Manusia Kesehatan.
Suharlina, S. (2018). Uji Kesesuaian Pemeriksaan Kehamilan Metode Strip Test Dengan Metode Aglutinasi. Prosiding Seminar Kesehatan Perintis, 1(1), 2622–2256. Retrieved from https://jurnal.upertis.ac.id/index.php/PSKP/article/view/73
Theofanakis, C., Drakakis, P., Besharat, A., and Loutradis, D. (2017). Human chorionic gonadotropin: The pregnancy hormone and more. International Journal of Molecular Sciences, 18(5), 1-8. doi: 10.3390/ijms18051059. DOI: https://doi.org/10.3390/ijms18051059
