The Activities Of Combination Citrus hystrix Peel Extract and Carica papaya Leaves Extract Against Candida albicans and Escherichia coli
Aktivitas Campuran Ekstrak Kulit Citrus hystrix dan Ekstrak Daun Carica papaya Terhadap Candida albicans dan Escherichia coli
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(1) Septarini Dian Anitasari Institut Keguruan dan Ilmu Pendidikan PGRI Jember
Indonesia
(2) * Dwi Nur Rikhma Sari
Institut Keguruan dan Ilmu Pendidikan PGRI Jember
Indonesia
(*) Corresponding Author
Abstract
Citrus hystrix Peel Extract and Carica papaya Leaves Extract contain several active components that can be used as antimicrobial compounds. The aim was to test a mixture of extracts from Citrus hystrix peel and Carica papaya leaves as growth inhibiting compounds for Candida albicans and Escherichia coli. This study used 4 levels of treatment, namely a combination of papaya leaves extract and orange fruit peel at concentrations of 0%, 25%, 50%, 75% and 100%. The research data were in the form of the diameter of the growth inhibition of Escherichia coli and Candida fungi, which were analyzed using the Kruskall-Wallis test at 5% confidence level and 5% Duncan's test. showed a significant difference between treatments, but the concentration of 100% showed better results to inhibit Escherichia coli (1.58 ± 0.28 d) and the growth of Candida albicans (1.53 ± 0.57b) compared to controls and other concentrations.
References
Anggara, E. D., Suhartanti, D., & Mursyidi, A. (2014).Uji aktivitas antifungi fraksi etanol infusa daun kepel (Stelechocarpus burahol, Hook F & Th.) terhadap Candida albicans. Prosiding Seminar Nasional & Internasional. Retrieved from https://jurnal.unimus.ac.id/index.php/psn12012010/article/view/1179
Ariyani, H., Nazemi, M., Hamidah, H., & Kurniati, M. (2018). Uji Efektivitas Antibakteri Ekstrak Kulit Limau (Cytrus Hystrix Dc) Terhadap Beberapa Bakteri. JCPS (Journal of Current Pharmaceutical Sciences), 2(1), 136-141. Retrieved from https://journal.umbjm.ac.id/index.php/jcps/article/view/210
Aulifa, D. L., & Aryantha, I. N. P. (2014). Aktivitas Anti Jamur Ekstrak Metanol Dari Tumbuhan Rempah-rempahan. Bionatura, 16(1), 10-15. Retrieved from http://jurnal.unpad.ac.id/bionatura/article/view/7554
Copriady, J., Yasmin E., & Hidayati. (2005). Isolasi dan Karakterisasi Senyawa Kumarin dari Ekstrak Kulit Jeruk Purut (Citrus hystrix D.C). Jurnal Biogenesis, 2(1), 13-15. Retrieved from https://nanopdf.com/download/isolasi-dan-karakterisasi-senyawa-kumarin-dari-kulit_pdf
Croxen, M. A., Law, R. J., Scholz, R., Keeney, K. M., Wlodarska, M., & Finlay, B. B. (2013). Recent advances in understanding enteric pathogenic Escherichia coli. Clinical microbiology reviews, 26(4), 822-880. doi: 10.1128/CMR.00022-13 DOI: https://doi.org/10.1128/CMR.00022-13
Duke, J. A. (2009). Dr. Duke’s Phytochemical and Ethnobotanical Databases. Retrieved from http://www.ars-Grin.Gov/Duke/
Febriani, T. H. (2014). Uji Daya Antifungi Jus Buah Pare (Momordica charantia L) Terhadap Daya Hambat Pertumbuhan Candida albicans Secara in vitro. Disertasi. Universitas Muhammadiyah Surakarta. Surakarta.
Gogna, N., Hamid, N., & Dorai, K. (2015). Metabolomic profiling of the phytomedicinal constituents of Carica papaya L. leaves and seeds by 1H NMR spectroscopy and multivariate statistical analysis. Journal of pharmaceutical and biomedical analysis, 115, 74-85. doi: 10.1016/j.jpba.2015.06.035 DOI: https://doi.org/10.1016/j.jpba.2015.06.035
Gunawan, D., & Mulyani, S. (2004). Ilmu Obat Alam (Farmakognosi). Jilid 1. Jakarta: Penebar Swadaya.
Hartini, S., & Mursyida, E. (2019). Efektivitas Pemberian Ekstrak Daun Pepaya (Carica Papaya L) Terhadap Pertumbuhan Bakteri Escherichia coli dan Shigella dysenteriae. Klinikal Sains: Jurnal Analis Kesehatan, 7(1), 8-17. doi: 10.36341/klinikal_sains.v7i1.590 DOI: https://doi.org/10.36341/klinikal_sains.v7i1.590
Hawkins, Bourne. (2011). Text book of Gynaecology. Edisi ke-15. USA: Elsevier Publication.
Illahiyyah, F. (2017). Uji Efektivitas Daya Hambat Antimikroba Minyak Jeruk Purut (Citrus hystrix DC) Terhadap Bakteri Streptococcus pyogenes dan Khamir Candida albicans Secara In Vitro. Disertasi. Universitas Brawijaya. Malang.
Iriano, A. (2008). Efektivitas antibakteri infusum Aloe vera terhadap porphyromonas gingivalis in vitro. Skripsi. Universitas Indonesia. Jakarta.
Jawetz, E., Melnick, J., & Adelberg, E. A. (2005). Mikrobiologi Kedokteran. Edisi ke-25. Jakarta: EGC.
Kusumaningtyas, E. (2015). Mekanisme Infeksi Candida albicans pada Permukaan Sel. Veteriner Lokakarya Nasional Penyakit Zoonosis, Bogor: Balai Penelitian. Retrieved from peternakan.litbang.pertanian.go.id/fullteks/lokakarya/lkzo05-48.pdf.
Lan-Phi, N. T., & Vy, T. T. (2015). Chemical composition, antioxidant and antibacterial activities of peels' essential oils of different pomelo varieties in the south of Vietnam. International Food Research Journal, 22(6), 2426-2431. Retrieved from http://www.ifrj.upm.edu.my/22%20(06)%202015/(35).pdf
Ogunjobi, A. A., & Ogunjobi, T. E. (2011). Comparative Study of Antibacterial Activities of Ethanol Extracts of the Bark and Seeds of Garcinia kola and Caricapapaya. African Journal of Biomedical Research, 14(2), 147-152. Retrieved from https://www.ajol.info/index.php/ajbr/article/view/95249
Roni, A., Maesaroh, M., & Marliani, L. (2019). Aktivitas antibakteri biji, kulit dan daun pepaya (Carica papaya L.) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Kartika: Jurnal Ilmiah Farmasi, 6(1), 29-33. doi: 10.26874/kjif.v6i1.134 DOI: https://doi.org/10.26874/kjif.v6i1.134
Rose, L. C., Suhaimi, H., & Mohamad, H., Rozaini, M. Z. H., & Taib, M. (2011). Preliminary evaluation on the antibacterial activities of Citrus hystrix oil emulsions stabilized by tween 80 and span 80. Int. J. Pharm. Pharm. Sci, 3(2), 209-211. Retrieved from https://www.researchgate.net/publication/289399193_Preliminary_evaluation_on_the_antibacterial_activities_of_citrus_hystrix_oil_emulsions_stabilized_by_tween_80_and_span_80
Shinta, D. Y., & Hartono, A. (2018). Uji Aktivitas Antimikroba Ekstrak Kulit Buah Naga (Hylocareus costarisensis) Terhadap E. coli, Staphylococcus aureus, dan Candida albicans. Sainstek: Jurnal Sains dan Teknologi, 9(1), 26-39. doi: 10.31958/js.v9i1.602 DOI: https://doi.org/10.31958/js.v9i1.602
Sugito, S., & Suwandi, E. (2017). Efektifitas Ekstrak Ethanol Daun Pepaya (Carica Papaya L) terhadap Pertumbuhan Bakteri Escherichia Coli dengan Metode Difusi. Jurnal Laboratorium Khatulistiwa, 1(1), 21-25. doi: 10.30602/jlk.v1i1.91 DOI: https://doi.org/10.30602/jlk.v1i1.91
Sousa, O. V., Del-Vechio-Vieira, G., Alves, M. S., Araújo, A. A., Pinto, M. A., Amaral, M. P., ... & Kaplan, M. A. (2012). Chemical composition and biological activities of the essential oils from Duguetialanceolata St. Hil. barks. Molecules, 17(9), 11056-11066. doi: 10.3390/molecules170911056 DOI: https://doi.org/10.3390/molecules170911056
Tadhfirah, F. (2010). Pengaruh Ekstrak Daun Pepaya (Caricapapaya L.) terhadap Pertumbuhan Shigella dysenteriae secara In Vitro. Skripsi. Universitas Brawijaya. Malang.
Tenaillon, O., Skurnik, D., Picard, B., & Denamur, E. (2010). The population genetics of commensal Escherichia coli. Nature reviews microbiology, 8(3), 207-217. doi: 10.1038/nrmicro2298 DOI: https://doi.org/10.1038/nrmicro2298
Tuntun, M. (2016).Uji Efektivitas Ekstrak Daun Pepaya (Carica papaya L.) Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Kesehatan, 7(3), 497-502. doi: 10.26630/jk.v7i3.235 DOI: https://doi.org/10.26630/jk.v7i3.235
Vijayakumar, M., Bharathidasan, R., & Prince, L. (2015). Antimicrobial Activity of Carica papaya L. Int J Arts Sci Res, 2(2), 37–43. Retrieved from http://www.ijasrjournal.com/article/ANTIMICROBIAL%20ACTIVITY%20OF%20CARICA%20PAPAYA%20L.pdf
Warsito, W., Noorhamdani, N., Sukardi, S., & Suratmo, S. (2017). Aktivitas antioksidan dan antimikroba minyak jeruk purut (Citrus hystrix DC.) dan komponen utamanya. Journal of Environmental Engineering and Sustainable Technology, 4(1), 13-18. doi: 10.21776/ub.jeest.2017.004.01.3 DOI: https://doi.org/10.21776/ub.jeest.2017.004.01.3
Wuryanti, W., &Murnah, M. (2009).Uji Ekstrak Bawang Bombay Terhadap Anti Bakteri Gram Negatif Pseudomonas aeruginosa Dengan Metode Difusi Cakram. Jurnal Sains dan Matematika, 17(3), 151-158. Retrieved from https://ejournal.undip.ac.id/index.php/sm/article/view/3280
Zubier, F., Bramono, K., Widaty, S., Nilasari, H., Louisa, M., & Rosana, Y. (2010). Efikasi sabun ekstrak sirih merah dalam mengurangi gejala keputihan fisiologis. Majalah Kedokteran Indonesia, 60(1), 9-14. Retrieved from https://adoc.pub/efikasi-sabun-ekstrak-sirih-merah-dalam-mengurangi-gejala-ke.html
Zukhri, S. (2016). Efektifitas Antibakteri Ekstrak Etanol Biji Pepaya (Carica papaya L) Terhadap Bakteri Escherichia coli. MOTORIK Jurnal Ilmu Kesehatan, 10(20), 21-30. Retrieved from http://jurnal.stikesmukla.ac.id/index.php/motor/article/view/219
