Medicra (Journal of Medical Laboratory Science/Technology)

Overview Of Anemia In Young Women Low Body Mass Index (Thin Category)

Annisa Rohmania Risna’im, Erni Yohani Mahtuti, Muhammad Masyhur, Faisal — Sat, 31 Dec 2022 00:00:00 +0000

In the period of growth and development, adolescents need more nutrients. Adolescents face many health problems due to biological and psychological changes as well as malnutrition. Anemia is a common health problem. Riskesdas data in 2018, in Indonesia there is a prevalence of anemia of 48.9%. Cases of anemia often occur in the age group of 15-24 years and 25-34 years. This study aims to determine the state of anemia with a lean body mass index in adolescent girls at SMK Madani Turen, Malang Regency. The research design used is descriptive quantitative with a cross sectional approach and a total of 101 respondents. The samples obtained had a lean body mass index of 24 people (23.76%) and the majority were known not to be anemic, namely 17 people (70.83%) and those who had anemia were 7 people (29.16%). The p value of the Fisher's exact test is 0.000 if the p value is <0.05, which means Ho is rejected, it can be concluded that there is a relationship between body mass index and Hb levels in female students at SMK Madani Turen.

Identification Of Soil Transmitted Helminth Using Formol Ether Sedimentation And ZnSO4 Solution Flotation Methods

Naura Silmy Taquillah, Erni Yohani Mahtuti, Muhammad Masyhur, Faisal — Sat, 31 Dec 2022 00:00:00 +0000

Soil transmitted helminths are parasitic groups of worms that live in countries with subtropical or tropical climates. In humans, when exposed to parasitic eggs or larvae, they can cause infection. Stool examination is divided into qualitative and quantitative examination. Qualitative are direct examination, flotation, tape, deposition, and thick preparations. Kato-Katz and Stoll are quantitative. The purpose of this study was to compare the results of STH identification using the Formol Ether Sedimentation Method and ZnSO₄ Solution Flotation with a total sample of 30 samples using purposive sampling. Data analysis with percentage tables and the Chi Square statistical correlation test The results of the analysis prove that the number of samples infected with soil transmitted Helminth with the Formol Ether Sedimentation Method and ZnSO₄ solution flotation is the same. With p value = 0.850, which means there is no significant difference between Formol Ether Sedimentation and ZnSO4 solution flotation.

Ability Analysis Of Waste Milkfish (Chanos chanos) As Alternative Medium Of Nutrient Agar On Escherichia coli And Staphylococcus aureus Growth

Gusti Naila Rachmawati Gufron, Pestariati, Syamsul Arifin — Sat, 31 Dec 2022 00:00:00 +0000

Waste milkfish got a lot from the residual processed milkfish production of Sidoarjo. The intestines, liver, and kidneys of waste milkfish contain protein, carbohydrates, and fat. The abundant availabilities were usable as a bacterial growth medium, including Nutrient Agar. Nutrient Agar has a high price and is produced by many foreign companies that can trigger a hard availability in laboratories of domestic healthcare, especially in frontier, outermost, and underdeveloped areas. This research aims to analyze the ability of waste milkfish as an alternative medium for Nutrient Agar on the growth of Escherichia coli and Staphylococcus aureus. The ability has been observed by the characteristics and number of bacterial colonies. This research was held in the bacteriology laboratory of the Medical Laboratory Technology Department, Health Polytechnic of Surabaya health ministry. The research type was True-experimental with a post-test-only control group design. Based on the preliminary test results, the waste milkfish mass variations were found in 7 grams, 7.5 grams, 8 grams, 8.5 grams, and 9 grams. Bacteria have been inoculated by the spread plate method, bacterial colony growth has been calculated by the Total Plate Count method. The data were analyzed by the Kruskal-Wallis test with a Sig. value <0.05 that there were differences in the number of Escherichia coli and Staphylococcus aureus colonies in milkfish waste media with various mass. The results showed that the best ability of waste milkfish as an alternative medium for Nutrient Agar on bacterial growth was found in 9 grams mass variation. the average number of Escherichia coli colonies were 186 × 10¹³ CFU/mL and Staphylococcus aureus colonies were 188 × 10¹³ CFU/mL.

Fixation Process With 10% KOH Immersion And Variation Of Heating Temperatures On The Quality Of Pediculus humanus capitis

Nurul Azizah, Erni Yohani Mahtuti, Faisal — Sat, 31 Dec 2022 00:00:00 +0000

The preparation of preserved preparations is one of the useful methods to determine the morphology of ectoparasites and insects whose manufacturing process goes through four stages starting with 10% KOH fixation, dehydration process, clearing process, and mounting process. The 10% KOH immersion serves to thin out the chitin layer that forms the exoskeleton, which is a thick covering on the body surface of Pediculus humanus capitis (human lice). The purpose of this study was to determine the quality of the preserved preparations of Pediculus humanus capitis by immersing them in 10% KOH at temperatures of 60°C, 70°C, 80°C, and 90°C for 30 minutes using the whole mount method, in order to observe the entire morphology of the species clearly. The sample used is Pediculus humanus capitis adult stage, as many as 24 tails. Observation of the quality of preserved preparations was assessed based on clarity, color quality, and morphological integrity. At a temperature of 60°C, two good qualities are obtained. Three good qualities are obtained at 70^oC. At 80°C, it is of good quality, and at 90°C, there is no good quality. The results of the study were then subjected to statistical tests using the Kruskall-Wallis test, which obtained a p value of 0.040<0.05, so that it could be interpreted that there was an influence of variations in heating temperature and 10% KOH immersion on the quality of Pediculus preserved preparations. humanus capitis. namely, by immersing 10% KOH at 70°C for 30 minutes, with complete morphological characteristics, transparent color, and a clear appearance.

Effectiveness Test of Okra Fruit (Abelmoschus esculentus) Extract on The Growth of Trichophyton rubrum

Bella Tasya Kimberly, Chylen Setiyo Rini — Sat, 31 Dec 2022 00:00:00 +0000

Dermatophytosis is an infectious disease of the skin caused by one of the pathogenic agents of the fungus Trichophyton. Trichophyton rubrum is a dermatophyte fungus that is included in the anthropophilic classification, where transmission occurs through direct human-to-human contact. This experiment was conducted to determine the effectiveness of giving okra fruit extract (Abelmoschus esculentus) to the growth of Trichophyton rubrum using well method, media that is used to do that is SDA (Sabouraud Dextrose Agar) with various concentrations of 25 %, 50%, 75%, 100%. Based on the results of phytochemical tests, the active compounds contained in okra fruits extract are alkaloids, flavonoids, saponins, steroid, triterpenoids, phenolics and tannins. Variations in concentration of okra fruit extract and negative control tested against Trichophyton rubrum did not show the formation of inhibition zones in the media. Meanwhile for positive control using 2% ketoconazole, an inhibition zone 35 mm was formed. This research showed that Trichophyton rubrum was resistant to okra fruit extract.

Toxicity Test of Ethanol Extract Lempuyang Gajah (Zingiber Zerumbet (L.) Roscoe Ex Sm.) on Bilirubin and Alkaline Phosphatase Levels in Mice (Mus Musculus)

Ririn Fatmawati, Jamilatur Rohmah — Sat, 31 Dec 2022 00:00:00 +0000

The lempuyang gajah plant (Zingiber zerumbet (L.) Roscoe ex Sm.) is a plant that is widely used as a treatment for desease. The purpose of this study was to determine the toxic effect of the ethanol extract of lempuyang gajah (Zingiber zerumbet (L.) Roscoe ex Sm.) on levels of bilirubin and alkaline phosphatase (ALP) in the liver of mice (Mus musculus). The type of research used is descriptive quantitative with laboratory experimental methods. The mice were divided into 5 groups including normal control, negative control, dose of 4000 kg^-1, 5000 kg^-1 and 6000 kg^-1. then observed the toxic symptoms, macroscopic, levels of bilirubin and alkaline phosphatase. The results of the phytochemical test showed that there were compounds of alkaloids, flavonoids, saponins, phenolics, steroids, triterpenoids and tannins. The results of macroscopic observations showed that the liver was still in a normal condition. The results of the One Way Anova test on bilirubin and alkaline phosphatase levels did not show any significant effect (p>0,05) influence between the normal control group, negative control, doses of 4000 kg^-1, 5000 kg^-1 and 6000 kg^-1, so it can be said that all experimental animals had the same condition.

Effect Of Infused White Dragon Fruit (Hylocereus undatus) Pell On Hemoglobin Level, Eritrosit Count, And Hematocrit Levels In Anemia White Rats

Syahrul Ardiansyah, Hanna Sielvi , Lailatul Handayani , Fitria Eka Wulandari — Sat, 31 Dec 2022 00:00:00 +0000

Anemia is a medical condition when the concentration of hemoglobin, erythrocytes, or hematocrit is less than average. The peels of white dragon fruit contains iron, vitamin C and flavonoids, which play an essential role as hemoglobin production. These flavonoids can increase erythropoiesis (erythrocyte formation process) and have immunostimulant effects. This research aimed to analyze the effect of giving peel undatus level infusion toward the number of hemoglobin, erythrocytes and hematocrit levels in anemia sufferer. Twenty-five male wistar rats given per oral injected with Natrium Nitrit before treatment. Wistar rats then grouped into five treatment groups, they are: the control group (without treatment), infusion of peels white dragon fruit with a concentration of 25%, 50%, 75%, and 100%. The results showed that there was a significant increase on the number of hemoglobin, erythrocytes and hematocrit levels of the rat after it was given with infusion of white dragon fruit peel. The conclution of this research is infusion of white dragon fruit peel increase the number of hemoglobin, erythrocytes and hematocrit levels.

The Effect Of Centrifugation Speed On The Results Of Urine Sediment Examination

Muslimah Uswatun Hasanah, Puspitasari Puspitasari — Sat, 31 Dec 2022 00:00:00 +0000

The process of centrifugation in urine examination is very important because it can be used in the manufacture of urine sediment. The purpose of this study was to determine the effect of centrifugation speed on the results of urine sediment examination. Centrifugation speeds used were 3000 rpm, 3500 rpm, 4000 rpm, and 4500 rpm using pathological urine samples. The samples used were 32. The research method used was an experimental laboratory at the Pusura Yos Sudarso clinical laboratory in Surabaya. The results of the study with a speed of 3000 rpm obtained an average of erythrocytes = 6.63/LPB, leukocytes = 13.63/LPB, and epithelial = 10.50/LPB. At a speed of 3500 rpm the average value of erythrocytes = 7.63/LPB, leukocytes = 15.13/LPB, and epithelium 11.63/PLB. At a speed of 4000 rpm the average value of erythrocytes = 8.63/LPB, leukocytes = 17.75/LPB and epithelial = 13.50/LPB. At a speed of 4500 rpm the average value of erythrocytes = 9.63/LPB, leukocytes = 20.50/LPB, and epithelial = 15.38/LPB. The results of the statistical test One Way Anova obtained p-value results of 0.001 < (0.05) in erythrocyte cells, p-value results for leukocytes 0.025 < (0.05), and p-value results for epithelial cells 0.002 < (0.05) means that there is an effect on the centrifugation speed on the results of urine sediment examination.

Relationship of Red Cell Distribution Width (RDW) To the Results Total Iron Binding Capacity (TIBC) In Chronic Kidney Failure Patients with Anemia

Devayanti Eka Safitri, Evy Diah Woelansari, Suhariyadi — Sat, 31 Dec 2022 00:00:00 +0000

Chronic kidney failure is caused by the body's inability to maintain metabolism and fluid balance due to progressive kidney function disorders that will trigger anemia. The cause of anemia in kidney failure is inflammation, which causes inhibition of iron release, resulting in a decrease in iron in the body. Signs of iron deficiency in chronic kidney failure are low levels of Total Iron Binding Capacity (TIBC) and in complete blood count; there is an increase in levels of Red Cell Distribution Width (RDW). This study aims to determine the relationship between Red Cell Distribution Width (RDW) and the results of Total Iron Binding Capacity (TIBC) in patients with chronic kidney failure with anemia. This study was conducted in January-April 2022, using a cross sectional method on 30 samples of patients with chronic kidney failure with anemia by examining a sample of patients at the Haji Regional General Hospital Surabaya. Most of the research results of the research subjects were male (n =16; 53,3%). The normal RDW is 33,3% (10/30), the high RDW is 66,7% (20/30), the low TIBC is 76,7% (23/30), and the normal TIBC is 23,3% (7/30). As much as 53,3% (16/30) for high RDW values with low TIBC. The result of the Pearson correlation test between RDW and TIBC was r = 0.014 (p = 0.940). Therefore, there is no significant relationship between RDW and TIBC in CKD patients with anemia at the Haji Surabaya Hospital.

Analysis Of Lead (Pb) Heavy Metal Content In Smoking Farmers

Desy Tamala, Galuh Ratmana Hanum — Sat, 31 Dec 2022 00:00:00 +0000

Farmers in the village of kedungrejo jombang in carrying out their activities some of them have a smoking habit where in cigarettes there are some heavy metal content such as lead (Pb). Lead (Pb) is a heavy metal that is toxic to human health. Lead can cause interference and damage to the kidney function system. The purpose of the study was to determine the relationship between lead heavy metals in the urine of actve and passive smokers in kedungrejo village. This research used the Atomic Absorption Spectrophotometer (AAS) method with 20 farmer urine samples. The results showed that lead heavy metal levels were below the threshold value of 0,15 mg/l. The results of Pearson correlation obtained p value = 0,021 ; r = 0,510. It can be concluded that there is a moderate relationship between heavy metals and urine of active and passive smokers of farmers.

The The Effect of Variations of Serum Examination Time Lapse on Electrolyte Levels of Potassium and Chloride

Nur Indah Nila Asri, Andika Aliviameita — Sat, 31 Dec 2022 00:00:00 +0000

Electrolyte is a chemical substance that dissociates in the liquid into charged particles of positive and negative ions. In the laboratory, conditions are often found that make an examination not immediately carried out after sampling. The purpose of this study was to determine the effect of variations in serum examination time intervals on Potassium and Chloride electrolyte levels for 0, 4, 8 and 24 hours at room temperature (20-25℃). This study used a laboratory experimental method with a sample of 32 samples. This research was conducted at the Bhayangkara Hospital Pusdik Brimob Watukosek Laboratory. Examination of Potassium and Chloride electrolyte levels using the OPTI CCA-TS Analyzer. The results of the One Way Anova test for Potassium electrolyte levels obtained a significance p value of = 0,199 meaning that there was no effect of variations in serum examination time intervals on Potassium electrolyte levels, while Chloride electrolyte levels obtained a significance value of p = 0,002 meaning that there is an effect of variations in serum examination time intervals on Chloride electrolyte levels.

Relationship of Leukocyte Examination to Intra Uterine Growth Restriction (IUGR) in Pregnant Women Experiencing Anemia

Andreas Putro Ragil Santoso, Nur Masruroh, Sisca Filia — Sat, 31 Dec 2022 00:00:00 +0000

Anemia in pregnant women will often occur because of increasing gestational age, the increasing need for nutrients in the form of iron in the body which increases during the 2nd to 3rd trimester of pregnancy. Anemia has an impact of around 14-62% on pregnant women including the occurrence of Intra Uterine Growth Restriction (IUGR) which is a condition that causes fetal growth to be stunted which is characterized by the size and weight of the baby that is not appropriate during pregnancy. The next thing to worry about is the emergence of infection if the patient has IUGR. The purpose of this study was to analyze the relationship of leukocytes to IUGR in pregnant women who are anemic with the parameters measured being leukocyte levels, IUGR values in anemic patients. The study was carried out in an analytic observational manner, then samples were obtained from 24 patients. Then, the patients filled out informed consent indicating that the patient was willing to be a research sample. Furthermore, the patient's blood was taken to measure leukocyte levels and based on the KIA book, IUGR levels were measured. Based on the above, it shows that the value of the results of the Spearman correlation test is 0.085 (p> 0.05). It can be concluded that there is no relationship between leukocyte levels and IUGR values in anemic patients at IBI Surabaya Hospital.