Medicra (Journal of Medical Laboratory Science/Technology)

The Effect Of Delay Time And Storage Temperature On The Results Of Urine Leukocyte Examination In Patients Of Urinary Tract Infection (UTI)

Rika Krisdianti, Puspitasari Puspitasari, Andika Aliviameita — Mon, 31 Jul 2023 00:00:00 +0000

Urinary Tract Infection (UTI) is a condition where there are microorganisms that multiply in the urinary tract causing inflammation and bacteriuria. The delay in the examination results in an error in diagnosis, the examination must be carried out >1 hour and a maximum of 2 hours after the voiding process at a temperature of 20-25℃ and the delay can be done a maximum of 4 hours at a temperature of 2-8℃. The study was conducted in June 2022. The study was conducted at SMK Muhammadiyah 1 Pandaan. UTI sampling at Sahabat Hospital in Suwayuwo Sukorejo. The purpose of this study was to determine the effect of delay time and storage temperature on the results of urine leukocyte examination in UTI patients. This study used an experimental method using 35 urine samples. The statistical test used the Two Way Anova test with a 95% confidence level and the inaccuracy limit of (α) = 0.05. Based on the results of the Two Way Anova test between storage temperature and delay in urine leukocytes, a significance value of 0.981 (p>0.05), it can be concluded that Ha is rejected and Ho is accepted, meaning that there is no interaction effect between the length of time delay and storage temperature on leukocyte results. urine in UTI patients.

Perineal Ectopic Testis in One and A Half Year Old Child As A Rare Congenital Anomaly: A Case Report

Prima Ardiansah Surya, Ashifa Hasna Faadilah, Sriyono — Mon, 31 Jul 2023 00:00:00 +0000

Ectopic testis is described as an abnormality of testicle development in which one or both testicles aren’t descending through the testicle normal pathways. Among all newborns with undescended testis, only 1% are diagnosed as ectopic testis. In this case, an 18 months old boy was presented to hospital with chief complaint of impalpable testis dextra. Phsical examination showed the right testis was not palpable in the scrotum and perineum. Ultrasonography examination showed a right testis in the perineum with normal volume and vascularization. Right orchidopexy was performed to correct the location of the testis. Early diagnostic of ectopic testis must be done through anamnesis, physical examination, and advanced examination. Limited faciliiesy and lack of parental knowledge make the patients come late to health facility. The conclusion of this study is knowledge among parents and skills of health workers concerning congenital anomaly especially undescensus testis should be improved.

Comparison of HCG Levels in Reagents Temperature 25°C and 8°C Using ELISA Method

Prisca Audra Telleng, Budi Santosa, Aprilia Indra Kartika — Mon, 31 Jul 2023 00:00:00 +0000

HCG hormone is a hormone produced by the placenta in early pregnancy with the aim of maintaining pregnancy. The Elisa method is a method for examining HCG levels which has the principle of specific antigen and antibody reactions. The work stage in the laboratory that has the biggest error is in the pre-analytic stage reaching 60%, one of which is the reagent temperature. The ELISA method recommends the reagent temperature to be stored at 2-8°C and when used at 25°C. Examination reagents Elisa method uses reagents that use enzymes in the examination. Enzymes are very sensitive to temperature. This study aims to determine the ratio of HCG levels at reagent temperatures of 25°C & 8° using the ELISA method. This type of research is analytic supported by experiments and literature studies. Serum samples of 6 pregnant women were taken from the Kedungmundu Public Health Center using a limited population count formula. The examination was carried out by comparing the reagents at a temperature of 25°C and 8°C using 6 serums of pregnant women and in duplicate. Data analysis only used primary data and statistical tests were carried out using normality, homogeneity and T-Test tests. The average result at a temperature of 25°C was 380.851 and the average temperature of 8°C was 321.99 and the T-test statistic test showed p value = 0.469 and 0.470. Based on the results of the study, it can be concluded that there is an insignificant difference in the concentration of HCG by comparing the reagents at 25°C and 8°C.

Potential of Seed Powder (Tamarindus indica L.) on Cholesterol Levels

Mon, 31 Jul 2023 00:00:00 +0000

ABSTRACT

Coronary heart disease (CHD) is the leading cause of death after stroke in all age groups at 12.9%. The main reason is the amount of cholesterol in the blood. Tamarind (Tamarindus indica) is a traditional herb that has the potential to lower cholesterol, because it contains flavonoids, saponins, foliphenols, pectin, organic acids, vitamin B and vitamin C that can lower cholesterol. The purpose of this study was to determine the effect of tamarind seed powder (Tamarindus indica) on cholesterol levels. This type of research is experimental with pre-test and post-test with control group research design. The population and sample of this study were a group of mice totaling 32 mice. The location of this research was conducted at the Animal Husbandry Center (PUSVETMA). Data collection procedures were carried out by examining the cholesterol levels of mice before and after treatment. The data obtained were followed up with normality test and independent t test. The results of the study using the unpaired t test gave the result of p=0.000 (p<0.05). The conclusion of this study is that there is an effect of seed powder (Tamarindus indica) on cholesterol levels.

Keyword : Seeds Tamarindus indica L., cholesterol levels, Mice (Mus musculus)

Analysis Of The Inhibitory Ability Of Spike Attachment Of The Delta Variant Of Sars Cov-2 With Ace2 By The Active Compound In Turmeric (Curcuma longa L.) In Silico

Pratasyah Liyaajul, Miftahul Mushlih, Chylen Setiyo Rini, Jamilatur Rohmah — Mon, 31 Jul 2023 00:00:00 +0000

Turmeric (Curcuma longa L.) is an herbal plant that has many benefits as a treatment, including during the COVID-19 pandemic, one of the mechanisms of inhibition of SARS CoV-2 is to inhibit the attachment of ACE2 with Spike. The binding of the spike protein to the ACE2 receptor will produce conformational changes in the S protein, this study was conducted using an in silico method (computational analysis) which aims to determine the potential efficacy of Turmeric and its effectiveness in inhibiting the Delta variant of SARS CoV-2. The active compound contained in Turmeric (Curcuma longa L. ) obtained from the KNApSAcK database To determine compounds that can have potential and have good effectiveness in inhibition of the Delta Variant of SARS CoV-2, an analysis was carried out by looking at the binding energy and conformation changes that occur at the sticky point in each compound. Three-dimensional structure of SARS CoV-2 Varian Delta downloaded from the Protein Data Bank with PDB code 7V8B. Based on the analysis carried out, it was found that the compound (E)-nuciferoll has the lowest binding energy value of -1212.59 kcal / mol and is located at the initial attachment but cannot change the conformation, but from the sticky point of the compound (E)-nuciferol lies in the initial attachment of RBD-ACE2.

Test The Effectiveness Of Japanese Papaya Leaf Extract (Cnidoscolus aconitifolius) On Aedes aegypti Larvae Mortality

Syahrul Ardiansyah, Faizatun Nafsi, Galuh Ratmana Hanum — Mon, 31 Jul 2023 00:00:00 +0000

Dengue hemorrhagic fever (DHF) is caused by the dengue virus which is transmitted through the bite of the Aedes aegypti mosquito. Prevention of DHF can be done by using larvicides. Synthetic chemical larvicides are considered to have side effects in long-term use and can cause environmental pollution. So we need an alternative larvicides with natural ingredients from plants, namely Japanese Papaya plants. This study aims to determine the effectiveness of Japanese Papaya (Cnidoscolus aconitifolius) leaf extract on the mortality of Aedes aegypti larvae. This type of research uses an experimental study design. This study used 6 treatments with 4 repetitions consisting of control without treatment, P1 (0.2%), P2 (0.4%), P3 (0.6%), P4 (0.8%), and P5 (1%). Observations were made for 48 hours. From the research results obtained LC50 of 1.095%. The results of statistical tests using One-Way ANOVA showed that there was a significant effect on the mortality of mosquito larvae.

Prevalence Of Helmint Infection In Cat’s Feces In Surabaya City

Mon, 31 Jul 2023 00:00:00 +0000

Cats are one of the most popular animals because of their cute and tame appearance. Just like other organism, cats can become infected worms if they are not kept clean. Cats infected with worms will initially lose their appetite, over time they will lose weight. Cats infected with worms, if kept and not treated continuously, can transmit to cat owners (humans). This study aims to determine the prevalence of parasitic worm infection in cat feces in the city of Surabaya, and to compare the prevalence of parasitic worm infection in domesticated cats with cats living wild in the city of Surabaya. The samples used in this study were 100 faecal samples, which were taken from 50 cats that live in the wild and from 50 live cats that are kept. Examination of faecal samples was carried out directly and indirectly using the saturated NaCl debris method, the data obtained will be presented descriptively and analyzed using the Chi-square test. Based on the results of the research conducted, the prevalence of worm infection in feral cat feces was 60%, while in domestic cats the prevalence was 10%. The types of worms that were found were Toxocara spp., Ancylostoma spp., Diphyllobothrium spp., and Trichuris spp. Based on the Chi-square test, it can be concluded that there is a significant relationship (P<0.05) between the prevalence of parasitic worm infection in domesticated cats and cats that live in the wild.