Medicra (Journal of Medical Laboratory Science/Technology) <p>This journal published by Medical Laboratory Technology Department, Health Science Faculty of Universitas Muhammadiyah Sidoarjo. Medicra journal published two times a year in July and December. The aim of this journal is to facilitate lecturers, researchers, and scholars for publishing the original research articles or review articles. Articles to be published on Medicra&nbsp;should follow the&nbsp;focus and scope of this journal. &nbsp;The article must have been edited according to the journal author guidelines before submitting. The scope of this journal are: Clinic Chemical,&nbsp;Hematology, Microbiology, Parasitology, Immunology, Food and beverage analysis Chemical, Molecular Diagnostics, Toxicology, Cytology, Histology, Epidemiology, Laboratory Management, and Laboratory Quality Control.</p> en-US (Andika Aliviameita, S.ST., M.Si.) (Pusat Pengembangan Publikasi Ilmiah (P3I) UMSIDA) Fri, 08 Mar 2024 09:08:47 +0000 OJS 60 Biochemical and Hematological Assessment in Patients With Thyroid Dysfunction <p>This investigation conducted for biochemical and hematological assessment in patients with thyroid dysfunction. The research was carried out at a laboratory in Baghdad, Iraq, and it is a case-control study. The sample was gathered from February 2023 through the years 2021 and 2022. About 250 individuals were a part of this study; 100 were hypothyroid, 100 were hyperthyroid, and 50 had normal thyroid function and complete blood counts. A total of three millilitres of whole blood and two millilitres of EDTA were obtained from each subject in an aseptic manner for the CBC. Also, 2 ml of blood was drawn for serum separation, these sera were used for evaluation T3, T4, TSH, AST, ALT, Urea and Creatinine by using kits purchased from Linear company, the procedures for all were done according to manufacturer instructions. The control group had considerably greater mean RBC, Hb, HCT, and MCH concentrations, while the hypothyroid group had significantly lower values. The hyperthyroid group showed no significant difference between MCV and MCH. There were no statistically significant MCHC results in either the hypothyroid or hyperthyroid groups compared to the control group. There was little difference in total lymphocyte and platelet counts between the control, hyperthyroid, and hypothyroid groups. Differential leukocyte count showed statistically significant differences between the hypothyroid and hyperthyroid groups. As seen in Table 2, the hypothyroid group had higher serum TSH levels (p&lt;0.05), in contrast to the hyperthyroid group which exhibited lower levels (p&lt;0.05). Alternatively, when contrasted with the control group, the hypothyroid group had lower T3 and T4 levels (p&lt;0.05), whereas the hyperthyroid group had higher levels (p&lt;0.05). Improvements in hepatic and kidney functions were seen in patients with hypo or hyperthyroidism as compared to the control group in this investigation. In conclusion, both hypothyroidism as well as hyperthyroidism affects on hematological and biomarkers for thyroid, liver and kidney</p> Nadia. H. Kadhum Copyright (c) 2024 Nadia. H. Kadhum Fri, 08 Mar 2024 00:00:00 +0000 The Protective Role of Nigela Sativa Volatile Oil on Antioxidant and Oxidative Stress Enzymes <p>Hepatotoxicity is the outcome of a paracetamol overdose.In this study, 40 adult male rats, weighing 180–260 gm and aged between 9 and 13 weeks, were given doses of Nigela sativa oil and their liver enzymes were examined to see how paracetamol affected them. The rats were kept in an environment at a temperature of 25 C<sup>o</sup> in an animal house. &nbsp;,There were four groups of rats, and food was provided for them. (G). (G1) The only injections given to the control group are food and regular saline (0.9% of the time). (G2) 200 mg/kg of N. sativa and (G3) 400 mg/kg of paracetamol Addition of 400 mg/kg body weight of paracetamol. supplying 300 mg/kg B.W. of N. sativa to (G4)400 milligrams/kg B.W. of aspirin. Rat G2, GPT 87.80 IUL, and GOT hepatic enzyme concentration (129.32 IUL) all exhibit significant increases in comparison to the control. Furthermore, the levels of the liver GSH enzyme in rats (G2 )1.24 IUL shown a substantial increase, whereas G3 ( GOT ) 91.9 IUL, (GPT )76.70 IUL, and group (G4) GOT 109.88 IUL and enzyme (GPT ) 55.66 IUL exhibited significant declines as compared with control. While MDA enzyme in rats G2 (0.259 IUL) shows a major increase in comparison to the control, rats G3 (0.139 IUL) and G4 (0.112) show a noticeable decrease in comparison to G2. While body weight indicates that G2's outcomes are significantly lower than those of the other three groups. Groups G3 (2.55 gm) and G4 (2.7 gm) demonstrated a significant rise in liver weight compared to controls and G2, whereas groups G2 (24.23 gm) demonstrated a significant increase in liver weight compared to controls and group G2. Male rats in groups G2 (1.7 gm) demonstrated a significant drop in liver weight compared to controls.</p> Ahmed Tawfeeq Ahmed, Inas Hazim Hameed, Walaa Hazim Hameed Copyright (c) 2024 Medicra (Journal of Medical Laboratory Science/Technology) Mon, 01 Jul 2024 00:00:00 +0000